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Image Search Results
Journal: Clinical cancer research : an official journal of the American Association for Cancer Research
Article Title: Ponatinib inhibits polyclonal drug-resistant KIT oncoproteins and shows therapeutic potential in heavily pretreated gastrointestinal stromal tumor (GIST) patients
doi: 10.1158/1078-0432.CCR-14-1397
Figure Lengend Snippet: Resistant cells recovered from N-ethyl-N-nitrosourea treated Ba/F3 KIT Ex11 (Δ557-558) cells, cultured with imatinib, sunitinib, regorafenib or ponatinib at the indicated concentrations. Each bar represents the relative frequency of the indicated KIT kinase domain secondary mutation, based on next generation sequencing data (reported mutation frequencies are a composite of both mutation incidence and cell number). ATP pocket residues are underlined in red and A-loop residues in blue. Mutagenesis data are shown from a representative experiment; similar results were obtained in three separate studies.
Article Snippet: Resistant cells were harvested, the KIT kinase domain PCR-amplified and analyzed by next
Techniques: Cell Culture, Mutagenesis, Next-Generation Sequencing